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HA-Tag Monoclonal Antibody(1B10)#44005

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Product Detail

Product NameHA-Tag Monoclonal Antibody(1B10)

Clone No.1B10

Host SpeciesMouse

ClonalityMonoclonal

IsotypeIgG1

ApplicationsWB IP IF

SpecificityThe antibody detects C-terminal, internal, and N-terminal HA-tag fusion proteins.

Immunogen TypePeptide

Immunogen DescSynthetic Peptide

Target NameHA-Tag

ConjugateUnconjugated

Concentration1mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

StorageStore at -20˚C/1 year

Application Details
Western blotting: 1:500-1:2000
Immunoprecipitation: 1:100
Immunofluorescence: 1:50-1:100
0.5ug HA fusion protein+ Primary antibody dilution at 1:10000
IP antibody use: 5ug HA Mouse IgG1 per ml Lysate, WB 1: 5000 1) untransfected 293 cell lysate 2) transfected 293 cell lysate with HA-tag fusion protein 3) IP (untransfted 293+anti-HA mAb+Protein G agarose) 4) IP (transfected 293+ normal Mouse IgG+Prote
IF analysis of 293 cells transfected with a HA-tag protein,1:2000 dilution (blue DAPI, red anti-HA)

If you have published an article using product 44005, please notify us so that we can cite your literature.

et al,Identification of c-di-GMP/FleQ-Regulated New Target Genes, Including cyaA, Encoding Adenylate Cyclase, in Pseudomonas putida. In mSystems on 2021 May 11 by Yujie Xiao, Haozhe Chen,et al..PMID:33975969 , (2021),
PMID: 33975969

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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