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C12orf51 Antibody#48079

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Product Detail

Product NameC12orf51 Antibody

Clone No.A2-F5-F10

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,IHC,ICC,FC

Species ReactivityHu, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesC12orf51 antibody
HECT domain-containing protein 4 antibody
HECTD4 antibody
KIAA0614 antibody
Probable E3 ubiquitin-protein ligase HECTD4 antibody

Accession NoSwiss-Prot#:Q9Y4D8

Uniprot Q9Y4D8

Calculated MW438/145/98/262 kDa

Formulation1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500
IHC: 1:50-1:100
ICC: 1:50

FC: 1:50-1:100
Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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