Product Detail
Product NameC12orf51 Antibody
Clone No.A2-F5-F10
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB,IHC,ICC,FC
Species ReactivityHu, Ms
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesC12orf51 antibody
HECT domain-containing protein 4 antibody
HECTD4 antibody
KIAA0614 antibody
Probable E3 ubiquitin-protein ligase HECTD4 antibody
Accession NoSwiss-Prot#:Q9Y4D8
Uniprot
Q9Y4D8
Calculated MW438/145/98/262 kDa
Formulation1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500
IHC: 1:50-1:100
ICC: 1:50
FC: 1:50-1:100
Western blot analysis of C12orf51 on SH-SY5Y (1) and A549 (2) using anti-C12orf51 antibody at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-C12orf51 antibody. Counter stained with hematoxylin.
ICC staining C12orf51 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining C12orf51 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of SH-SY5Y cells with C12orf51 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
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