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Location： Home > Mouse Monoclonal Antibodies > TMEM177 Antibody

TMEM177 Antibody#48097

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Product Detail

Product NameTMEM177 Antibody

Clone No.A6-A11-9

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesTM177_HUMAN antibody
Tmem177 antibody
Transmembrane protein 177 antibody

Accession NoSwiss-Prot#:Q53S58

Uniprot Q53S58

Gene ID 80775;

Calculated MW34 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200

Western blot analysis of TMEM177 on recombinant protein using anti-TMEM177 antibody at 1/1,000 dilution.
ICC staining TMEM177 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TMEM177 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining TMEM177 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded hman stamoch tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus muscle tissue using anti-TMEM177 antibody. Counter stained with hematoxylin.
A transmembrane protein (TMEM) is a type of integral membrane protein that spans the entirety of the biological membrane to which it is permanently attached. Many transmembrane proteins function as gateways to permit the transport of specific substances across the biological membrane. They frequently undergo significant conformational changes to move a substance through the membrane. Transmembrane proteins are polytopic proteins that aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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