Product Detail
Product NameAlpha smooth muscle Actin Antibody
Clone No.A5-E6
Host SpeciesMouse
ClonalityMonoclonal
PurificationProG affinity purified
ApplicationsWB, ICC, IHC, FC
Species ReactivityHu,Ms
Immunogen DescThis antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to a region of alpha smooth muscle Actin.
ConjugateUnconjugated
Other Namesa actin antibody
AAT6 antibody
ACTA_HUMAN antibody
ACTA2 antibody
Actin alpha 2 smooth muscle aorta antibody
Actin aortic smooth muscle antibody
Actin, aortic smooth muscle antibody
ACTSA antibody
ACTVS antibody
Alpha 2 actin antibody
Alpha actin 2 antibody
Alpha cardiac actin antibody
Alpha-actin-2 antibody
Cell growth inhibiting gene 46 protein antibody
Cell growth-inhibiting gene 46 protein antibody
GIG46 antibody
Growth inhibiting gene 46 antibody
MYMY5 antibody
Accession NoSwiss-Prot#:P62736
Uniprot
P62736
Gene ID
59;
Calculated MW42 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000
IHC: 1:200
ICC: 1:200
Western blot analysis of alpha smooth muscle Actin on NIH-3T3 (1), human heart (2) and mouse heart (3) using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
ICC staining alpha smooth muscle Actin (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.
If you have published an article using product 48104, please notify us so that we can cite your literature.
et al,Enhancing Cutaneous Wound Healing Based on Human Induced Neural Stem Cell-derived Exosomes
, (2022),
PMID:
36506346
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