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Alpha-tubulin Antibody#48111

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Product Detail

Product NameAlpha-tubulin Antibody

Clone No.A8-6

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu,Ms,Rt, zebrafish

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesTUBA1 antibody
TUBA1A antibody
TUBA2 antibody
TUBA3 antibody
TUBA3C antibody
TUBA4A antibody
Tubulin, alpha 1a antibody
Tubulin, alpha 3c antibody
Tubulin, alpha 4a antibody

Accession NoSwiss-Prot#:P68366

Uniprot P68366

Gene ID 7277;

Calculated MW50 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:5,000

ICC: 1:500

Western blot analysis on PC12 cell lysates using anti-α-tubulin mouse mAb.
ICC staining α-tubulin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

If you have published an article using product 48111, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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