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Location： Home > Mouse Monoclonal Antibodies > Glucose Transporter GLUT4 Antibody

Glucose Transporter GLUT4 Antibody#48116

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Product Detail

Product NameGlucose Transporter GLUT4 Antibody

Clone No.3-A10

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC

Species ReactivityHu, Ms

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesGlucose transporter GLUT 4 Glucose transporter type 4 Glucose transporter type 4 insulin responsive GLUT 4 GLUT-4 GLUT4 GTR4_HUMAN Insulin responsive glucose transporter type 4 insulin-responsive kug SLC 2A4 SLC2A4 solute carrier family 2 (facilitated glucose transporter) member 4 Solute carrier family 2 member 4 Solute carrier family 2, facilitated glucose transporter member 4

Accession NoSwiss-Prot#:P14672

Uniprot P14672

Gene ID 6517;

Calculated MW55 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:2,000-1:5,000

ICC: 1:100-1:200

Western blot analysis on NIH/3T3 cell lysates using anti- Glut4 mouse mAb.
ICC staining Glut4 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Under conditions of low insulin, most GLUT4 is sequestered in intracellular vesicles in muscle and fat cells. Insulin induces a rapid increase in the uptake of glucose by inducing the translocation of GLUT4 from these vesicles to the plasma membrane. As the vesicles fuse with the plasma membrane, GLUT4 transporters are inserted and become available for transporting glucose, and glucose absorption increases. Muscle contraction stimulates muscle cells to translocate GLUT4 receptors to their surfaces. This is especially true in cardiac muscle, where continuous contraction can be relied upon; but is observed to a lesser extent in skeletal muscle.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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