Product Detail
Product NameNF-κB p105/p50 Antibody
Clone No.A2-7
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC, IHC
Species ReactivityHu,Ms
Immunogen DescThis antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) corresponding to NF-κB p105/p50.
ConjugateUnconjugated
Other NamesDKFZp686C01211 antibody
DNA binding factor KBF1 antibody
DNA binding factor KBF1 EBP1 antibody
DNA-binding factor KBF1 antibody
EBP 1 antibody
EBP-1 antibody
EBP1 antibody
KBF1 antibody
MGC54151 antibody
NF kappa B antibody
NF kappaB antibody
NF kappabeta antibody
NF kB1 antibody
NFkappaB antibody
NFKB 1 antibody
NFKB p105 antibody
NFKB p50 antibody
Nfkb1 antibody
NFKB1_HUMAN antibody
Nuclear factor kappa B DNA binding subunit antibody
Nuclear factor kappa-B, subunit 1 antibody
Nuclear factor NF kappa B p105 subunit antibody
Nuclear factor NF kappa B p50 subunit antibody
Nuclear factor NF-kappa-B p50 subunit antibody
Nuclear factor of kappa light chain gene enhancer in B cells 1 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody
p105 antibody
p50 antibody
p84/NF-kappa-B1 p98 antibody
Transcription factor NFKB1 antibody
Accession NoSwiss-Prot#:P19838
Uniprot
P19838
Gene ID
4790;
Calculated MW50kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:200
Western blot analysis of NF-κB p105/p50 on MCF-7 cell lysates.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 antibody. Counter stained with hematoxylin.
ICC staining NF-κB p105/p50 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators.
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