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Eftud2 Antibody#48121

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Product Detail

Product NameEftud2 Antibody

Clone No.1-D10-A1

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu,Ms,Rt

Immunogen Descpeptide

ConjugateUnconjugated

Other Names116 kDa antibody
116 kDa U5 small nuclear ribonucleoprotein component antibody
EFTUD2 antibody
Elongation factor Tu GTP binding domain containing 2 antibody
Elongation factor Tu GTP-binding domain-containing protein 2 antibody
hSNU114 antibody
MFDGA antibody
MFDM antibody
SNRNP116 antibody
Snrp116 antibody
Snu114 antibody
SNU114 homolog antibody
U5 116KD antibody
U5 small nuclear ribonucleoprotein component antibody
U5 snRNP specific protein, 116 kD antibody
U5 snRNP specific protein, 116 kDa antibody
U5 snRNP-specific protein antibody
U5-116 kDa antibody
U5-116KD antibody
U5S1_HUMAN antibody

Accession NoSwiss-Prot#:O08810

Uniprot O08810

Gene ID 20624;

Calculated MW116 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb. Positive control:
Lane 1: Jurkat
Lane 2: NIH/3T3
Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-EFTUD2 Mouse mAb.
Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-EFTUD2 Mouse mAb.
Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-EFTUD2 Mouse mAb.
Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-EFTUD2 Mouse mAb.
Immunocytochemical staining of SH-SY-5Y cells using anti-EFTUD2 Mouse mAb
Immunocytochemical staining of SW480 cells using anti-EFTUD2 Mouse mAb.
Flow cytometric analysis of MCF-7 cells with EFTUD2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ncubation with primary antibody; black). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Spliceosomes are multi-protein complexes that are composed of snRNPs (small nuclear ribonucleoproteins) and a variety of associated protein factors, all of which work in concert to regulate the splicing of pre-mRNA. Snrp116, also known as EFTUD2 (elongation factor Tu GTP binding domain containing 2) or Snu114, is a 972 amino acid protein that localizes to the nucleus and belongs to the GTP-binding elongation factor family. Existing as a component of the multi-protein U5 snRNP spliceosome complex, Snrp116 plays an important role in pre-mRNA splicing, as well as in the recycling of spliceosomal snRNPs. The gene encoding Snrp116 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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