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β-Actin Antibody#48139

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Product Detail

Product Nameβ-Actin Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationImmunogen affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu,Ms,Rt,Zebrafish, bamboo

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesA26C1A antibody
A26C1B antibody
ACTB antibody
ACTB_HUMAN antibody
Actin beta antibody
Actin cytoplasmic 1 antibody
Actin, cytoplasmic 1, N-terminally processed antibody
Actx antibody
b actin antibody
Beta cytoskeletal actin antibody
Beta-actin antibody
BRWS1 antibody
E430023M04Rik antibody
MGC128179 antibody
PS1TP5 binding protein 1 antibody
PS1TP5BP1 antibody

Accession NoSwiss-Prot#:P60709

Uniprot P60709

Gene ID 60;

Calculated MW42kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-5,000



Western blot analysis of β-actin on different lysates using anti-β-actin antibody at 1/1,000 dilution.

Positive control��

Lane 1: NIH/3T3  

Lane 2: Hela  

Lane 3: PC12      

Lane 4: Zebrafish


Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting.

If you have published an article using product 48139, please notify us so that we can cite your literature.

et al,Insulin receptor substrate-1 inhibits high-fat diet-induced obesity by browning of white adipose tissue through miR-503. In FASEB J on 2020 Sep by Xiao-Fei Man, Nan Hu,,et al..PMID:32721050 , (2020),
PMID: 32721050
et al,Knockout of the BRAP homolog in mice leads to abnormal tracheal ciliaInFEBS LettOn2023 NovbyHui Wang 1, Suhui Zuo et al..PMID: 37715941 , (2023),
PMID: 37715941
et al,ANKRD22 promotes M2 polarization in lung adenocarcinoma macrophages via the glycolytic pathway. In Chem Biol Drug Des on 2024 Jan by Jiangbo Cao, Hongwei Zhang, et al..PMID:38230786 , (2024),
PMID: 38230786
et al,Gingerenone A induces ferroptosis in colorectal cancer via targeting suppression of SLC7A11 signaling pathway. In Biomed Pharmacother on 2024 Nov by Hongyu Xiao, Chen Chen, et al..PMID: 39393329 , (2024),
PMID: 39393329
et al,Jin-Gui-Shen-Qi Wan ameliorates diabetic retinopathy by inhibiting apoptosis of retinal ganglion cells through the Akt/HIF-1α pathway , (2023),
PMID: 37828620
et al,Exposure to Manganese Induces Autophagy–Lysosomal Pathway Dysfunction-Mediated Tauopathy by Activating the cGAS–STING Pathway in the Brain , (2025),
PMID:
et al,Neuroprotective effects of lixisenatide against propagation of α-synuclein pathology in Parkinson's disease. , (2025),
PMID: 40145958
More
et al,Ditan Decoction ameliorates vascular dementia-induced cognitive dysfunction through anti-ferroptosis via the HIF1α pathway: Integrating network pharmacology and experimental validation. , (2025),
PMID: 39978449

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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