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GAPDH Antibody#48142

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Product Detail

Product NameGAPDH Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationImmunogen affinity purified

ApplicationsWB

Species ReactivityHu,Ms,Rt,Zebrafish,Rabbit

Immunogen Descpeptide

ConjugateUnconjugated

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Accession NoSwiss-Prot#:P04406

Uniprot P04406

Gene ID 2597;

Calculated MW36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:2,000



Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/1,000 dilution.

Positive control:

Lane 1: Ribbit liver tissue  

Lane 2: Zebrafish

Lane 3: NCCIT                

Lane 4: Mouse heart tissue

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets.

If you have published an article using product 48142, please notify us so that we can cite your literature.

et al,Mycn ameliorates cardiac hypertrophy-induced heart failure in mice by mediating the USP2/JUP/Akt/β-catenin cascade. In BMC Cardiovasc Disord on 2024 Jan 31 by Weinian Gao, Na Guo,et al..PMID:38297207 , (2024),
PMID: 38297207

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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