Product Detail
Product NameALAS2 Antibody
Clone No.12-D
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, IP, IF, IHC(P)
Species ReactivityHu, Ms, Rt
Immunogen Descpeptide
ConjugateUnconjugated
Other Names5 @aminolevulinate synthase erythroid specific antibody 5 aminolevulinate synthase 2 antibody 5 aminolevulinate synthase 5 aminolevulinate synthase 2 antibody 5 aminolevulinate synthase erythroid specific mitochondrial antibody 5 aminolevulinic acid synthase 2 antibody 5 aminolevulinic acid synthase antibody 5-aminolevulinate synthase antibody 5-aminolevulinic acid synthase 2 antibody Alas 2 antibody ALAS antibody ALAS E antibody ALAS, erythroid antibody ALAS-E antibody Alas2 antibody ALASE antibody Aminolevulinate delta synthase 2 antibody Aminolevulinic acid synthase 2, erythroid antibody ANH1 antibody ASB antibody Delta ALA synthase 2 antibody Delta ALA synthetase antibody Delta aminolevulinate synthase 2 antibody Delta aminolevulinate synthase antibody Delta-ALA synthase 2 antibody Delta-aminolevulinate synthase 2 antibody Erythroid specific ALAS antibody erythroid-specific antibody FLJ93603 antibody HEM0_HUMAN antibody mitochondrial antibody OTTHUMP00000023388 antibody OTTHUMP00000023389 antibody OTTMUSP00000020679 antibody RP23-338A17.1 antibody SIDBA1 antibody XLDPP antibody XLEPP antibody XLSA antibody
Accession NoSwiss-Prot#:P22557
Uniprot
P22557
Gene ID
212;
Calculated MW60 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:100-1:1,000
IHC: 1:50-500
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
Western blot analysis of ALAS-E expression in non-transfected (A)and human ALAS-E transfected (B) 293T whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.
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