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Location： Home > Mouse Monoclonal Antibodies > ALCAM/CD166 Antibody

ALCAM/CD166 Antibody#48272

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Product Detail

Product NameALCAM/CD166 Antibody

Clone No.7C2

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IP, IF, IHC(P)

Species ReactivityHu, Ms, Rt

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesActivated leukocyte cell adhesion molecule antibody
ALCAM antibody
ALCAM protein antibody
CD 166 antibody
CD166 antibody
CD166 antigen antibody
CD166_HUMAN antibody
FLJ3851 antibody
FLJ38514 antibody
MEMD antibody
MGC71733 antibody

Accession NoSwiss-Prot#:Q13740

Uniprot Q13740

Gene ID 214;

Calculated MW100-105 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at 4˚C

Application Details

WB: 1:100-1:1,000
IHC: 1:50-500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Western blot analysis of ALCAM expression in SK-N-SH whole cell lysate.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing membrane staining of respiratory epithelial cells.

Activated leukocyte cell adhesion molecule (ALCAM), expressed on activated leukocytes, T cells, B cells and monocytes, is a member of the immunoglobulin superfamily (IgSF) and identified as a CD6 ligand. CD6 is a type I membrane protein in the scavenger receptor cysteine rich protein superfamily that acts in T cell adhesion and costimulation. ALCAM mediates thymocyte-thymic epithelial cell adhesion via homophilic (ALCAM-ALCAM) and heterophilic (ALCAMCD6) interactions involving a previously undescribed protein-protein interaction between a member of the scavenger receptor cysteine rich protein superfamily and the immunoglobulin superfamily.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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