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Location： Home > Mouse Monoclonal Antibodies > BrdU (Bu20a) Antibody

BrdU (Bu20a) Antibody#48275

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Product Detail

Product NameBrdU (Bu20a) Antibody

Clone No.1G1

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsIF, IHC(P), FCM

Species ReactivityBromodeoxyuridine

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesBromodeoxyuridine antibody
BUdr antibody

Accession NoSwiss-Prot#:

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at 4˚C

Application Details


IHC: 1:50-500


FC: 1 μg per 1 x 106 cells

Immunofluorescence staining of methanol-fixed BrdU-labeled HeLa cells showing nuclear localization.
Intracellular FCM analysis of fixed and permeabilized untreated (dotted blue histogram) and BrdU treated (solid orange histogram) K-562 cells. Green line histogram represents the isotype control, normal mouse IgG1.

The halogenated pyrimidine thymidine analog bromodeo-xyuridine (BrdU) is incorporated into newly synthesized DNA strands of S-phase cells and is useful for estimating the fraction of cells in S-phase. Additionally, the analysis of the uptake of BrdU is a reliable method to quantitate the degree of DNA-synthesis. BrdU is also useful for studying sister chromatid exchange and to isolate nascent DNA. UV-induced excision-repair synthesis is one method for incorporating BrdU into cellular DNA. Anti-BrdU antibodies bind to the exposed BrdU in single-stranded DNA after a hydrochloric acid denaturation step or nuclease digestion. Protease antigen recovery is necessary for most tissues or cells fixed with crosslinking agents such as formalin but may decrease the specificity of BrdUrd immunodetection. The monoclonal antibody Bu20a against BrdU stains BrdU incorporated into the nulcei of a wide range of proliferating cell types including human tumors growing in nude mice and tonsil lymphoid.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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