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Location： Home > Mouse Monoclonal Antibodies > Granzyme B Antibody

Granzyme B Antibody#48298

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Product Detail

Product NameGranzyme B Antibody

Clone No.4G9

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IP, IF, IHC(P)

Species ReactivityHu, Ms, Rt

Immunogen DescAmino acids 1-247 representing full length granzyme B of human origin.

ConjugateUnconjugated

Other NamesC11 antibody
Cathepsin G like 1 antibody
Cathepsin G-like 1 antibody
CCPI antibody
CGL 1 antibody
CGL1 antibody
CSP B antibody
CSPB antibody
CTLA-1 antibody
CTLA1 antibody
CTSGL1 antibody
Cytotoxic serine protease B antibody
Cytotoxic T lymphocyte associated serine esterase 1 antibody
Cytotoxic T lymphocyte proteinase 2 antibody
Cytotoxic T-lymphocyte proteinase 2 antibody
Fragmentin 2 antibody
Fragmentin-2 antibody
GRAB_HUMAN antibody
Granzyme 2 antibody
Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1) antibody
Granzyme B antibody
Granzyme-2 antibody
GranzymeB antibody
GRB antibody
Gzmb antibody
Hlp antibody
Human lymphocyte protein antibody
Lymphocyte protease antibody
Protease, serine, B antibody
SECT antibody
T cell serine protease 1-3E antibody
T-cell serine protease 1-3E antibody

Accession NoSwiss-Prot#:P10144

Uniprot P10144

Gene ID 3002;

Calculated MW32 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:100-1:1,000
IHC: 1:50-500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
Western blot analysis of granzyme B expression in non-transfected(A)and human granzyme B transfected(B)293T whole cell lysates.
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in red pulp.
Granzyme A and granzyme B are serine proteases that mediate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Both granzyme A and granzyme B are synthesized as inactive proenzymes, and they are stored within cytolytic granules and released by effector cells during degranulation. In activated CTLs, granzyme A and granzyme B are processed and activated by cathepsin C, and they then function to induce apoptosis by two distinct pathways. Granzyme B proteolytically cleaves and activates members of the caspase family of cysteine proteases, including caspase-3, caspase-6, caspase-7 and caspase-9. When cleaved, these caspases assemble into active holoenzymes that then mediate apoptosis through a defined proteolytic cascade involving nuclear lamins and PARP (poly ADP ribose polymerase). Granzyme A mediates the activation of apoptosis by inducing single-strand DNA breaks, membrane perturbation and nuclear condensations in an alternative pathway that is independent from caspase activation or the caspase proteolytic cascade.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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