Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Mouse Monoclonal Antibodies > MDM2 Antibody

MDM2 Antibody#48314

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameMDM2 Antibody

Clone No.3G2

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IP, IF, IHC(P)

Species ReactivityHu, Ms, Rt

Immunogen DescAmino acids 154-167 of MDM2 of human origin.

ConjugateUnconjugated

Other NamesACTFS antibody
Double minute 2 protein antibody
E3 ubiquitin-protein ligase Mdm2 antibody
Hdm 2 antibody
Hdm2 antibody
HDMX antibody
MDM 2 antibody
MDM2 antibody
MDM2 oncogene E3 ubiquitin protein ligase antibody
Mdm2 p53 E3 ubiquitin protein ligase homolog antibody
Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody
MDM2_HUMAN antibody
MDM2BP antibody
Mouse Double Minute 2 antibody
MTBP antibody
Murine Double Minute Chromosome 2 antibody
Oncoprotein Mdm2 antibody
p53 Binding Protein Mdm2 antibody
p53-binding protein Mdm2 antibody
Ubiquitin protein ligase E3 Mdm2 antibody
Ubiquitin protein ligase E3 Mdm2 antibody

Accession NoSwiss-Prot#:Q00987

Uniprot Q00987

Gene ID 4193;

Calculated MW90 kDa

Concentration2mg/ml

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:100-1:1,000
IHC: 1:50-1:500

IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Western blot analysis of MDM2 expression in U-2OS (A), A-673 (B) and RAW 264.7 (C) whole cell lysates.

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

If you have published an article using product 48314, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors