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YAP1 Antibody#48350

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Product Detail

Product NameYAP1 Antibody

Clone No.3G1

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IP, IF

Species ReactivityHu, Ms, Rt

Immunogen DescAmino acids 294-323 within an internal region of YAP of human origin.

ConjugateUnconjugated

Other Names65 kDa Yes associated protein antibody
65 kDa Yes-associated protein antibody
COB1 antibody
YAp 1 antibody
YAP 65 antibody
YAP antibody
YAP1 antibody
YAP1_HUMAN antibody
YAP2 antibody
YAP65 antibody
yes -associated protein delta antibody
Yes associated protein 1 65kDa antibody
Yes associated protein 1 antibody
Yes associated protein 2 antibody
yes associated protein beta antibody
YKI antibody
Yorkie homolog antibody

Accession NoSwiss-Prot#:P46937

Uniprot P46937

Gene ID 10413;

Calculated MW65kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at 4˚C

Application Details

WB: 1:100-1:1,000


IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

Western blot analysis of YAP expression in non-transfected 293T (A), human YAP transfected 293T (B) and Caco-2 (C) whole cell lysates.

The Yes-associated protein, otherwise known as YAP, is a 14-3-3-bindingmolecule that was originally recognized by virtue of its ability to bind to theSH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylationof a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. Thehighly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation,cell cycle progression and apoptosis by binding intracellular phosphoproteinsinvolved in signal transduction. YAP may link events at the plasmamembrane and cytoskeleton to inhibition of transcription in the nucleus in amanner regulated by 14-3-3 proteins. YAP shares homology with the WWdomain of TAZ, transcriptional co-activator with PDZ-binding motif, whichfunctions as a transcriptional co-activator by binding to the PPXY motif presentin transcription factors. YAP is expressed at high levels in the lung, placenta,prostate, ovary and testis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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