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Cytokeratin-7 Antibody#48356

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Product Detail

Product NameCytokeratin-7 Antibody

Clone No.3-G3-D8

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC

Species ReactivityHu

Immunogen Descpeptide

ConjugateUnconjugated

Other NamesCK 7 antibody
CK-7 antibody
CK7 antibody
Cytokeratin 7 antibody
Cytokeratin-7 antibody
D15Wsu77e antibody
K2C7 antibody
K2C7_HUMAN antibody
K7 antibody
Keratin 7 antibody
Keratin 7, type II antibody
Keratin type II cytoskeletal 7 antibody
Keratin, 55K type II cytoskeletal antibody
Keratin, simple epithelial antibody
Keratin, simple epithelial type I, K7 antibody
Keratin, type II cytoskeletal 7 antibody
Keratin-7 antibody
Krt2-7 antibody
KRT7 antibody
MGC11625 antibody
MGC129731 antibody
MGC3625 antibody
Sarcolectin antibody
SCL antibody
Type II mesothelial keratin K7 antibody
Type-II keratin Kb7 antibody

Accession NoSwiss-Prot#:P08729

Uniprot P08729

Gene ID 3855;

Calculated MW51 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000
IHC: 1:200


Western blot analysis of Cytokeratin-7 on different cell lysates using anti- Cytokeratin-7 antibody at 1/1000 dilution. Positive?control:
Lane 1: MCF-7
Lane 2: A549
Lane 3: Hela
Lane 4: HepG2
Lane 5: SKBR3 Lane6: SKOV3
Lane 7: SW1990
Immunohistochemical analysis of paraffin-embedded human lung?carcinoma tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Cytokeratin-7 antibody. Counter stained with hematoxylin.
Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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