Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Mouse Monoclonal Antibodies > Sequestosome-1 Antibody

Sequestosome-1 Antibody#48357

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NameSequestosome-1 Antibody

Clone No.10-E10

Host SpeciesMouse

ClonalityMonoclonal

PurificationPeptide affinity purified.

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic peptide (KLH-coupled) within human SQSTM1 40-100 aa.

ConjugateUnconjugated

Other NamesA170 antibody
DMRV antibody
EBI 3 associated protein of 60 kDa antibody
EBI 3 associated protein p60 antibody
EBI3 associated protein of 60 kDa antibody
EBI3 associated protein p60 antibody
EBI3-associated protein of 60 kDa antibody
EBIAP antibody
FTDALS3 antibody
MGC127197 antibody
ORCA antibody
OSF-6 antibody
Osi antibody
OSIL antibody
Oxidative stress induced like antibody
p60 antibody
p62 antibody
p62B antibody
Paget disease of bone 3 antibody
PDB 3 antibody
PDB3 antibody
Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa antibody
Phosphotyrosine independent ligand for the Lck SH2 domain p62 antibody
Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa antibody
PKC-zeta-interacting protein antibody
Protein kinase C-zeta-interacting protein antibody
Sequestosome 1 antibody
Sequestosome-1 antibody
SQSTM 1 antibody
SQSTM_HUMAN antibody
Sqstm1 antibody
STAP antibody
STONE14 antibody
Ubiquitin binding protein p62 antibody
Ubiquitin-binding protein p62 antibody
ZIP 3 antibody
ZIP antibody
ZIP3 antibody

Accession NoSwiss-Prot#:Q13501

Uniprot Q13501

Gene ID 8878;

Calculated MW62 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:200
ICC: 1:200

FC: 1 μg per 1 x 106 cells
Western blot analysis of SQSTM1?on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution. Positive control: </br>Lane1: Hela </br>Lane2: HepG2

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.

ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometric analysis of Hela?cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.

Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. It may play a role in titin/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. P62 may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.

If you have published an article using product 48357, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors