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Location： Home > Mouse Monoclonal Antibodies > GAPDH Antibody

GAPDH Antibody#48358

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Product Detail

Product NameGAPDH Antibody

Clone No.5-E10

Host SpeciesMouse

ClonalityMonoclonal

PurificationProL affinity purified

ApplicationsWB, ICC

Species ReactivityHu,Ms,Rt,Zebrafish,rabbit

Immunogen Descpeptide

ConjugateUnconjugated

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Accession NoSwiss-Prot#:P04406

Uniprot P04406

Gene ID 2597;

Calculated MW36 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:5,000-1:10,000

ICC: 1:200

Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution.


Positive control:

Lane 1: HepG2

Lane 2: Hela

Lane 3: PC12

Lane 4: NIH/3T3

Lane 5: MCF-7

Lane 6: Rabbit liver

Lane 7:Zebrafish


ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

If you have published an article using product 48358, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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