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Location： Home > Mouse Monoclonal Antibodies > EP-CAM Antibody

EP-CAM Antibody#48359

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Product Detail

Product NameEP-CAM Antibody

Clone No.3-E1-E2

Host SpeciesMouse

ClonalityMonoclonal

PurificationProL affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu,Ms

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other Names17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
Cell surface glycoprotein Trop 2 antibody
Cell surface glycoprotein Trop-1 antibody
CO 17A antibody
CO17 1A antibody
CO17A antibody
DIAR5 antibody
EGP 2 antibody
EGP antibody
EGP2 antibody
EGP314 antibody
EGP40 antibody
Ep CAM antibody
Ep-CAM antibody
EPCAM antibody
EPCAM_HUMAN antibody
EpCAM1 antibody
Epithelial cell adhesion molecule antibody
Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
Epithelial cell surface antigen antibody
Epithelial cellular adhesion molecule antibody
Epithelial glycoprotein 1 antibody
Epithelial glycoprotein 314 antibody
Epithelial glycoprotein antibody
ESA antibody
GA733 1 antibody
GA733 2 antibody
GA733-2 antibody
gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
gp4 antibody
hEGP 2 antibody
hEGP314 antibody
HNPCC8 antibody
Human epithelial glycoprotein 2 antibody
KS 1/4 antigen antibody
KS1/4 antibody
KSA antibody
Ly74 antibody
Lymphocyte antigen 74 antibody
M1S 1 antibody
M1S2 antibody
M4S1 antibody
Major gastrointestinal tumor associated protein GA733 2 antibody
Major gastrointestinal tumor-associated protein GA733-2 antibody
mEGP314 antibody
Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
Membrane component, chromosome 4, surface marker 1 antibody
Membrane component, chromosome 4, surface marker antibody
MIC18 antibody
MK 1 antibody
Protein 289A antibody
TACD1 antibody
TACSTD1 antibody
TROP1 antibody
Tumor associated calcium signal transducer 1 antibody
Tumor associated calcium signal transducer 2 precursor antibody
Tumor-associated calcium signal transducer 1 antibody

Accession NoSwiss-Prot#:P16422

Uniprot P16422

Gene ID 4072;

Calculated MW35 kDa

Concentration2 mg/mL

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

WB: 1:1,000-1:2,000
IHC: 1:200-1:500
ICC: 1:100-1:200

FC: 1:100-1:200

Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.


Positive control:

Lane1: Hela

Lane2: NIH/3T3

Lane3: MCF-7


Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained withhematoxylin.
ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. Specificity/Source: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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