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PCNA Antibody#48360

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Product Detail

Product NamePCNA Antibody

Clone No.A6-G11

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC

Species ReactivityHu, Ms, Rt

Immunogen Descrecombinant protein

ConjugateUnconjugated

Other NamesATLD2 antibody
cb16 antibody
Cyclin antibody
DNA polymerase delta auxiliary protein antibody
etID36690.10 antibody
fa28e03 antibody
fb36g03 antibody
HGCN8729 antibody
MGC8367 antibody
Mutagen-sensitive 209 protein antibody
OTTHUMP00000030189 antibody
OTTHUMP00000030190 antibody
PCNA antibody
Pcna/cyclin antibody
PCNA_HUMAN antibody
PCNAR antibody
Polymerase delta accessory protein antibody
Proliferating cell nuclear antigen antibody
wu:fa28e03 antibody
wu:fb36g05 antibody

Accession NoSwiss-Prot#:P12004

Uniprot P12004

Gene ID 5111;

Calculated MW29 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000
IHC: 1:200


FC:1:100
Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution. Positive control:
Line 1: L929
Line 2 :MCF-7
Line 3:PC12
Line 4:Raji
Line 5:F9
Line 6:A549
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
PCNA (Proliferating cell nuclear antigen) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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