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CD133 Antibody#48362

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Product Detail

Product NameCD133 Antibody

Clone No.D2-B1

Host SpeciesMouse

ClonalityMonoclonal

PurificationPeptide affinity purified

ApplicationsIHC,ICC

Species ReactivityHu, Ms

Immunogen DescPeptide

ConjugateUnconjugated

Other NamesAC133 antibody
Antigen AC133 antibody
CD133 antibody
CORD12 antibody
Hematopoietic stem cell antigen antibody
hProminin antibody
MCDR2 antibody
MSTP061 antibody
OTTHUMP00000217744 antibody
OTTHUMP00000217745 antibody
OTTHUMP00000217746 antibody
PROM1 antibody
PROM1_HUMAN antibody
Prominin I antibody
Prominin like 1 antibody
Prominin like protein 1 precursor antibody
Prominin mouse like 1 antibody
Prominin-1 antibody
Prominin-like protein 1 antibody
Prominin1 antibody
PROML1 antibody
RP41 antibody
STGD4 antibody

Accession NoSwiss-Prot#:O43490

Uniprot O43490

Gene ID 8842;

Calculated MWPredicted band size 97 kDa

Formulation1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

IHC: 1:50-1:200
ICC: 1:100-1:500

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD133 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD133 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.
ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ICC staining CD133 (green) in N2A cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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