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Location： Home > Mouse Monoclonal Antibodies > Anti-Taq DNA Polymerase Antibody

Anti-Taq DNA Polymerase Antibody#48369

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Product Detail

Product NameAnti-Taq DNA Polymerase Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationProG affinity purified

ApplicationsAntibody-mediated hot start PCR

Immunogen DescTaq DNA Polymerase

ConjugateUnconjugated

StorageStore at -20˚C

Application Details



 

Application of the hot start PCR using anti-Taq DNA Polymerase antibody
Lane1: Taq DNA Polymerase
Lane2: Taq DNA Polymerase + Anti-Taq DNA Polymerase antibody
Lane3: Taq DNA Polymerase + Control antibody

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. Anti-Taq Antibody is an ideal tool for hot-start PCR with Taq DNA polymerase. The Anti-Taq Antibody binds to Taq DNA polymerase and arrests the activity of Taq DNA Polymerase, preventing non-specific and primer dimer amplification resulted from non-specific priming at ambient temperature for the duration of time prior to PCR thermal cycling. During the initial denaturing step in PCR thermal cycling, the Anti-Taq Antibody is denatured and the Taq DNA polymerase is then released, thus regaining its full DNA polymerase activity. The result indicates that anti-Taq DNA Polymerase antibody increases the specificity and sensitivity of the PCR.

If you have published an article using product 48369, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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