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CUB domain-containing protein 1 Antibody#48377

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Product Detail

Product NameCUB domain-containing protein 1 Antibody

Clone No.B2-E6

Host SpeciesMouse

ClonalityMonoclonal

PurificationProtein affinity purified

ApplicationsWB,IHC,ICC

Species ReactivityHu, Ms, Rt

Immunogen DescRecombinant protein within Human CDCP1

ConjugateUnconjugated

Other Names9030022E12Rik antibody
AA409659 antibody
CD 318 antibody
CD_antigen=CD318 antibody
CD318 antibody
CD318 antigen antibody
CDCP 1 antibody
Cdcp1 antibody
CDCP1_HUMAN antibody
CUB domain containing protein 1 antibody
CUB domain-containing protein 1 antibody
E030027H19Rik antibody
FLJ13772 antibody
FLJ22375 antibody
FLJ22969 antibody
Membrane glycoprotein gp140 antibody
MGC31813 antibody
OTTHUMP00000164120 antibody
OTTHUMP00000209568 antibody
RGD1305578 antibody
SIMA 135 antibody
SIMA135 antibody
Subtractive immunization M plus HEp3 associated 135 kDa protein antibody
Subtractive immunization M plus HEp3-associated 135 kDa protein antibody
Transmembrane and associated with src kinases antibody
TRASK antibody

Accession NoSwiss-Prot#:Q9H5V8

Uniprot Q9H5V8

Gene ID 64866;

Calculated MW93 kDa

Concentration1ug/ul

FormulationPBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Application Details

WB 1:1000

Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution.
Lane 1: SW480 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: NIH/3T3 cell lysate (no heat)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-2: 24 seconds; Lane 3: 10 seconds; 4-20% SDS-PAGE gel.
Western blot analysis of CDCP1 on different lysates at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HCT 116 cell lysate treated with deglycosylation
Lysates/proteins at 14 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 93/130 kDa (Glycosylated)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.

CDCP1 (CUB-domain-containing protein 1) contains three extracellular CUB domains, a transmembrane domain, and two putative cytoplasmic tyrosine phosphorylation sites. Phosphorylation of the gp140 and p80 proteins is mediated by Src family kinases at various tyrosine residues, including Tyr 734. PTP family members mediate the desphosphorylation of CDCP1. The conversion of gp140 to p80 prolongs the phosphorylation state, which may affect signaling in epithelial wounds. CDCP1 acts as a marker for hematopoetic cells and also exhibits high expression in metastatic colon and breast tumors.

If you have published an article using product 48377, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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