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CAMK2G Antibody#48383

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Product Detail

Product NameCAMK2G Antibody

Clone No.10C-G1

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC, FC

Species ReactivityHu, Rt

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesCalcium/calmodulin dependent protein kinase II alpha antibody
Calcium/calmodulin dependent protein kinase II beta antibody
Calcium/calmodulin dependent protein kinase II delta antibody
Calcium/calmodulin dependent protein kinase II gamma antibody
Calcium/calmodulin-dependent protein kinase type II subunit alpha antibody
CaM kinase II alpha antibody
CaM kinase II antibody
CaM kinase II beta antibody
CaM kinase II delta antibody
CaM kinase II gamma antibody
CaM kinase II subunit alpha antibody
CaMK-II subunit alpha antibody
CAMK2 antibody
Camk2a antibody
CAMK2B antibody
CAMK2D antibody
CAMK2G antibody
CAMKA antibody
KCC2A_HUMAN antibody

Accession NoSwiss-Prot#:Q13555

Uniprot Q13555

Gene ID 818;

Calculated MW63 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:200-1:500


FC: 1:50-1:100
Western blot analysis of CaMKII gamma on human CaMKII gamma recombinant protein using anti- CaMKII gamma antibody at 1/1,000 dilution.
Western blot analysis of CaMKII gamma on HEK293 (1) and CaMKII gamma -hIgGFc transfected HEK293 (2) cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution.
Western blot analysis of CaMKII gamma on different cell lysate using anti- CaMKII gamma antibody at 1/1,000 dilution. Positive control�� Line1: PC-12 Line2: Jurkat Line3: T47D Line4:HepG2
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- CaMKII gamma antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Jurkat cells with CaMKII gamma antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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