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PRDM5 Antibody#48384

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Product Detail

Product NamePRDM5 Antibody

Clone No.12C-D4

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, FC

Species ReactivityHu, Ms

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesBCS2 antibody
PFM 2 antibody
PFM2 antibody
PR domain containing 5 antibody
PR domain containing protein 5 antibody
PR domain zinc finger protein 5 antibody
PR domain-containing protein 5 antibody
PRDM 5 antibody
PRDM5 antibody
PRDM5 protein antibody
PRDM5_HUMAN antibody

Accession NoSwiss-Prot#:Q9NQX1

Uniprot Q9NQX1

Gene ID 11107;

Calculated MW73 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:1,000-1:2,000

ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of PRDM5 on human PRDM5 recombinant protein using anti-PRDM5 antibody at 1/1,000 dilution.
Western blot analysis of PRDM5 on HEK293 (1) and PRDM5-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM5 antibody at 1/1,000 dilution.
ICC staining PRDM5 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PRDM5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
A cDNA of PRDM5 was isolated based upon its homology to the PR domain of PRDM2. The gene encodes an open reading frame of 630 amino acids and contains a PR domain in the NH-terminal region followed by 16 zinc finger motifs. Through radiation hybrid analysis, PRDM5 was mapped to human chromosome 4q27, a region thought to contain tumor suppressor genes for ovarian, breast, lung, liver, colon, and other cancers. The gene has a CpG island promoter and is silenced in human breast, ovarian, and liver cancers. Upon infection of tumor cells, a recombinant adenovirus expressing PRDM5 causes G2/M arrest and apoptosis, suggesting that inhibition of PRDM5 may be involved in carcinogenesis.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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