Product Detail
Product NameRab3a Antibody
Clone No.D1-12
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesRab 3A antibody
RAB 3A member RAS oncogene family antibody
Rab3a antibody
RAB3A member RAS oncogene family antibody
RAB3A_HUMAN antibody
RAS associated protein RAB 3A antibody
RAS associated protein RAB3A antibody
Ras related protein Rab 3A antibody
Ras related protein Rab3A antibody
Ras-related protein Rab-3A antibody
Accession NoSwiss-Prot#:P20336
Uniprot
P20336
Gene ID
5864;
Calculated MW25 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
FC: 1:100-1:200
Western blot analysis of Rab3a on human Rab3a recombinant protein using anti-Rab3a antibody at 1/1,000 dilution.
Western blot analysis of Rab3a on HEK293 (1) and Rab3a-hIgGFc transfected HEK293 (2) cell lysate using anti-Rab3a antibody at 1/1,000 dilution.
Flow cytometric analysis of Hela cells with Rab3a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possiblity that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab family have been identified, each of which is found at a particular stage of a membrane transport pathway.
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