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Location： Home > Mouse Monoclonal Antibodies > RAP1GAP Antibody

RAP1GAP Antibody#48393

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Product Detail

Product NameRAP1GAP Antibody

Clone No.B6-G7

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesKIAA0474 antibody
Rap1 GTPase activating protein 1 antibody
RAP1 GTPase activating protein antibody
Rap1 GTPase-activating protein 1 antibody
RAP1GA1 antibody
Rap1ga1 protein antibody
Rap1GAP antibody
Rap1GAP1 antibody
RAP1GAPII antibody
RAPGAP antibody
RPGP1_HUMAN antibody

Accession NoSwiss-Prot#:P47736

Uniprot P47736

Gene ID 5909;

Calculated MW73 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000



FC: 1:50-1:100
Western blot analysis of RAP1GAP on human RAP1GAP recombinant protein using anti-RAP1GAP antibody at 1/1,000 dilution.
Western blot analysis of RAP1GAP on HEK293 (1) and RAP1GAP-hIgGFc transfected HEK293 (2) cell lysate using anti-RAP1GAP antibody at 1/1,000 dilution.
Flow cytometric analysis of A431 cells with RAP1GAP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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