Product Detail
Product NameEPN1 Antibody
Clone No.F9-F7
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesEH domain binding mitotic phosphoprotein antibody
EH domain-binding mitotic phosphoprotein antibody
EPN 1 antibody
Epn1 antibody
EPN1_HUMAN antibody
EPS 15 interacting protein 1 antibody
EPS-15-interacting protein 1 antibody
Epsin 1 antibody
Epsin-1 antibody
Accession NoSwiss-Prot#:Q9Y6I3
Uniprot
Q9Y6I3
Gene ID
29924;
Calculated MW60 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
FC: 1:50-1:100
Western blot analysis of EPN1 on human EPN1 recombinant protein using anti-EPN1 antibody at 1/1,000 dilution.
Western blot analysis of EPN1 on HEK293 (1) and EPN1-hIgGFc transfected HEK293 (2) cell lysate using anti-EPN1 antibody at 1/1,000 dilution.
Flow cytometric analysis of A431 cells with EPN1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Epsin 1 (EPN1) is an endocytic accessory protein, with significant similarity to the Xenopus mitotic phosphoprotein MP90. Epsin 1 interacts with Eps15 (the α subunit of the Clathrin adaptor AP2), Clathrin and other accessory proteins. The mitotic phosphorylation of these proteins may be one of the mechanisms by which the invagination of Clathrin-coated pits is blocked in mitosis. Both epsin and Eps15, like other cytosolic components of the synaptic vesicle endocytic machinery, undergo constitutive phosphorylation and depolarization-dependent dephosphorylation in nerve terminals. Epsin 1 also contributes to the mechanism of Clathrin-vesicle-dependent endocytosis. The human Epsin 1 protein contains an epsin N-terminal homology (ENTH) region and a single Clathrin-binding (LVDLD) motif. Epsin 1 localizes to the leading edge of a vesicular coated pit where the membrane is being actively bent.
If you have published an article using product 48395, please notify us so that we can cite your literature.
Yes
