Product Detail
Product NameCHRNA7 Antibody
Clone No.G1-F10
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, IHC, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other Names7nAChR antibody
a7 nicotinic acetylcholine receptor antibody
acetylcholine receptor, neuronal nicotinic, alpha-7 subunit antibody
ACHA7_HUMAN antibody
AChR antibody
Acra7 antibody
alpha 7 neuronal nicotinic acetylcholine receptor antibody
alpha-7 nicotinic cholinergic receptor subunit antibody
alpha7 antibody
cholinergic receptor, neuronal nicotinic, alpha polypeptide 7 antibody
cholinergic receptor, nicotinic, alpha 7 (neuronal) antibody
cholinergic receptor, nicotinic, alpha 7 antibody
cholinergic receptor, nicotinic, alpha polypeptide 7 antibody
CHRNA7 antibody
CHRNA7-2 antibody
NACHRA7 antibody
neuronal acetylcholine receptor protein, alpha-7 chain antibody
Neuronal acetylcholine receptor subunit alpha-7 antibody
Accession NoSwiss-Prot#:P36544
Uniprot
P36544
Gene ID
1139;89832;
Calculated MW56 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
FC: 1:50-1:100
Western blot analysis of CHRNA7 on human CHRNA7 recombinant protein using anti-CHRNA7 antibody at 1/1,000 dilution.
Western blot analysis of CHRNA7 on HEK293 (1) and CHRNA7-hIgGFc transfected HEK293 (2) cell lysate using anti-CHRNA7 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CHRNA7 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-CHRNA7 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of SH-SY5Y cells with CHRNA7 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Members of the ligand-gated ion channel receptor family are characterized by their fast transmitting response to neurotransmitters. Two important members of this family are the nicotinic acetylcholine and glutamate receptors, both of which are composed of five homologous subunits forming a transmembrane aqueous pore. These transmembrane receptors change conformation in response to their cognate neurotransmitter. Nicotinic acetylcholine receptors (AChRs) are found at the postsynaptic membrane of the neuromuscular junction and bind acetylcholine molecules, allowing ions to move through the pore. Glutamate receptors are found in the postsynaptic membrane of cells in the central nervous system. The activity that is generated at the synapse by the binding of acetylcholine is terminated by acetylcholinesterase, an enzyme that rapidly hydrolyzes acetylcholine. AChRα7, also known as NACHRA7, CHRNA7-2 or CHRNA7, is a 502 amino acid multi-pass membrane protein existing as a homopentamer and interacts with RIC-3, a nicotinic acetylcholine receptor (nAChR)-associated protein.
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