Product Detail
Product NamePRDM1 Antibody
Clone No.B6-F1
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesB Lymphocyte Induced Maturation Protein 1 antibody
Beta interferon gene positive regulatory domain I binding factor antibody
Beta-interferon gene positive regulatory domain I-binding factor antibody
BLIMP-1 antibody
BLIMP1 antibody
Positive Regulatory Domain I Binding Factor 1 antibody
Positive regulatory domain I-binding factor 1 antibody
PR Domain Containing 1 antibody
PR domain containing 1 with ZNF domain antibody
PR domain containing 1 with ZNF domain isoform 2 antibody
PR domain containing protein 1 antibody
PR domain zinc finger protein 1 antibody
PR domain-containing protein 1 antibody
PRDI BF1 antibody
PRDI binding factor 1 antibody
PRDI-BF1 antibody
PRDI-binding factor 1 antibody
PRDM 1 antibody
Prdm1 antibody
PRDM1_HUMAN antibody
Accession NoSwiss-Prot#:O75626
Uniprot
O75626
Gene ID
639;
Calculated MW92 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
ICC: 1:200-1:1,000
FC: 1:100-1:200
Western blot analysis of PRDM1 on human PRDM1 recombinant protein using anti-PRDM1 antibody at 1/1,000 dilution.
Western blot analysis of PRDM1 on HEK293 (1) and PRDM1-hIgGFc transfected HEK293 (2) cell lysate using anti-PRDM1 antibody at 1/1,000 dilution.
ICC staining PRDM1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Raji cells with PRDM1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.
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