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PLCG1 Antibody#48404

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Product Detail

Product NamePLCG1 Antibody

Clone No.D11-C2

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu, Rt, Monkey

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
Inositoltrisphosphohydrolase antibody
Monophosphatidylinositol phosphodiesterase antibody
NCKAP3 antibody
Phosphatidylinositol phospholipase C antibody
Phosphoinositidase C antibody
Phosphoinositide phospholipase C antibody
Phosphoinositide phospholipase C-gamma-1 antibody
Phospholipase C 148 antibody
Phospholipase C gamma 1 antibody
Phospholipase C-gamma-1 antibody
Phospholipase C-II antibody
PLC gamma 1 antibody
PLC II antibody
PLC-148 antibody
PLC-gamma-1 antibody
PLC-II antibody
PLC1 antibody
PLC148 antibody
Plcg1 antibody
PLCG1_HUMAN antibody
PLCgamma1 antibody

Accession NoSwiss-Prot#:P19174

Uniprot P19174

Gene ID 5335;

Calculated MW148 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:100-1:200
Western blot analysis of PLCG1 on human PLCG1 recombinant protein using anti-PLCG1 antibody at 1/1,000 dilution.
Western blot analysis of PLCG1 on HEK293 (1) and PLCG1-hIgGFc transfected HEK293 (2) cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.
Western blot analysis of PLCG1 on different cell lysate using anti-PLCG1 antibody at 1/1,000 dilution.? Positive control�� Line1: Hela Line2: A431 Line3: C6 Line4: NIH/3T3 Line5: COS7 Line6: HCT116
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using anti-PLCG1 antibody. Counter stained with hematoxylin.
ICC staining PLCG1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with PLCG1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Phospholipase C-gamma 1 (PLC g1) is an isozyme of the phosphoinositide-specific PLC family, which occupies a central role in hormonal signal transduction pathways and is a substrate for the epidermal growth factor receptor tyrosine kinase. Following activation of TrkB, PLC g1 is phosphorylated on Tyrosine 783, Tyrosine 771 and Tyrosine 1253. Tyrosine 783 lies just downstream of the kinase domain in a relatively short sequence motif characteristic of the Trk family of protein-tyrosine kinase receptors. The sequence around Tyrosine 783 fits a consensus sequence for binding PLC g1. PLC g1 also forms a complex with TrkB consistent with the possibility that one of the TrkB autophosphorylation sites provides a binding site for the PLC g1 SH2 domains, as is the case for other receptor protein-tyrosine kinases.

If you have published an article using product 48404, please notify us so that we can cite your literature.

et al,Pyroptosis: a novel signature to predict prognosis and immunotherapy response in gliomas. In Hum Cell on 2022 Nov by Guiying He, Zhimin Chen, et al..PMID:36129672 , (2022),
PMID: 36129672

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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