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Location： Home > Mouse Monoclonal Antibodies > CDH11 Antibody

CDH11 Antibody#48405

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Product Detail

Product NameCDH11 Antibody

Clone No.A6-C12

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesCAD11 antibody
CAD11_HUMAN antibody
Cadherin 11 antibody
Cadherin 11 type 2 antibody
Cadherin 11 type 2 OB cadherin (osteoblast) antibody
Cadherin-11 antibody
Cdh11 antibody
CDHOB antibody
OB antibody
OB-cadherin antibody
OBcadherin antibody
OSF-4 antibody
OSF4 antibody
Osteoblast cadherin antibody

Accession NoSwiss-Prot#:P55287

Uniprot P55287

Gene ID 1009;

Calculated MW88 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of CDH11 on human CDH11 recombinant protein using anti-CDH11 antibody at 1/1,000 dilution.
Western blot analysis of CDH11 on HEK293 (1) and CDH11-hIgGFc transfected HEK293 (2) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.
Western blot analysis of CDH11 on MCF-7 (1), Jurkat (2) and HEK293 (3) cell lysate using anti-CDH11 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CDH11 antibody. Counter stained with hematoxylin.
ICC staining CDH11 (green) and Actin filaments (red) in HL-7702 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with CDH11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Cadherins are calcium dependent cell adhesion proteins, a family of Ca++-dependent adhesion molecules that influence cell-cell binding and are critical to the maintenance of tissue structure and morphogenesis. OB-cadherin (osteoblast-cadherin, cadherin-11, OSF-4) has two forms, OB-cadherin-1 and OB-cadherin-2. OB-cadherin-2 has a truncated cytoplasmic domain, missing amino acids 694-796. Both OB-cadherins are expressed in osteoblastic cell lines with low expression seen in lungs, testis and brain. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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