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SK2 Antibody#48406

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Product Detail

Product NameSK2 Antibody

Clone No.E1-C5

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsICC, IHC, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names3 prime phosphoadenosine 5 prime phosphosulfate synthase 2 antibody
3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
ATP sulfurylase/adenosine 5' phosphosulfate kinase antibody
ATP SULFURYLASE/ADENOSINE 5-PRIME PHOSPHOSULFATE KINASE 2 antibody
ATP sulfurylase/APS kinase 2 antibody
ATPSK2 antibody
Bifunctional 3' phosphoadenosine 5' phosphosulfate synthase 2 antibody
Bifunctional 3' phosphoadenosine 5' phosphosulfate synthethase 2 antibody
PAPS synthase 2 antibody
PAPS synthetase 2 antibody
PAPSS 2 antibody
PHOSPHOADENOSINE-PHOSPHOSULFATE SYNTHETASE 2 antibody
SK 2 antibody
SK2 antibody
Sulfurylase kinase 2 antibody

Accession NoSwiss-Prot#:Q9NRA0

Uniprot Q9NRA0

Gene ID 56848;

Calculated MW69 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details

IHC: 1:200-1:1000
ICC: 1:200-1:1000

FC: 1:50-1:100
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- SPHK2 antibody. Counter stained with hematoxylin.
ICC staining SPHK2 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with SPHK2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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