Product Detail
Product NameKDM3A Antibody
Clone No.C2-G10
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesDKFZp686A24246 antibody
DKFZp686P07111 antibody
JHDM2A antibody
JHMD2A antibody
JmjC domain-containing histone demethylation protein 2A antibody
JMJD1 antibody
JMJD1A antibody
Jumonji C domain containing histone demethylase 2A antibody
Jumonji domain containing 1 antibody
Jumonji domain containing 1A antibody
Jumonji domain containing protein 1A antibody
Jumonji domain-containing protein 1A antibody
Kdm3a antibody
KDM3A_HUMAN antibody
KIAA0742 antibody
Lysine (K) specific demethylase 3A antibody
Lysine-specific demethylase 3A antibody
Testis specific protein A antibody
TSGA antibody
Accession NoSwiss-Prot#:Q9Y4C1
Uniprot
Q9Y4C1
Gene ID
55818;
Calculated MW147 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
ICC: 1:50-1:200
FC: 1:100-1:200
Western blot analysis of KDM3A on human KDM3A recombinant protein using anti-KDM3A antibody at 1/1,000 dilution.
Western blot analysis of KDM3A on HEK293 (1) and KDM3A-hIgGFc transfected HEK293 (2) cell lysate using anti-KDM3A antibody at 1/1,000 dilution.
ICC staining KDM3A (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with KDM3A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
JMJD1A (Jumonji domain containing 1A), also known as TSGA (testis-specific protein A), JMJD1, KDM3A, JHDM2A (JMJC domain-containing histone demethylation protein 2A) or JHMD2A, is a member of the JHDM2 histone demethylase family of proteins that is predominantly expressed in testis. Containing one JMJC domain and a C-terminal C2HC4 zinc finger, JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and a-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.
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