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Location： Home > Mouse Monoclonal Antibodies > XRN2 Antibody

XRN2 Antibody#48415

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Product Detail

Product NameXRN2 Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, IHC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names5' 3' exoribonuclease 2 antibody
DHM1 like protein antibody
DHP protein antibody
XRN 2 antibody

Accession NoSwiss-Prot#:Q9H0D6

Uniprot Q9H0D6

Gene ID 22803;

Calculated MW109 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:200-1:500


Western blot analysis of XRN2 on human XRN2 recombinant protein using anti-XRN2 antibody at 1/1,000 dilution.
Western blot analysis of XRN2 on HEK293 (1) and XRN2-hIgGFc transfected HEK293 (2) cell lysate using anti-XRN2 antibody at 1/1,000 dilution.
Western blot analysis of XRN2 on different cell lysate using anti-XRN2 antibody at 1/1,000 dilution. Positive control�� Lane1: ?Raw264.7 Lane2: HEK293 Lane3: NTERA-2 Lane4: LNcap Lane5: HepG2 Lane6: HEK293 Lane7: Hela?
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XRN2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti- XRN2 antibody. Counter stained with hematoxylin.
Degradation of mRNA is a critical aspect of gene expression that occurs via the exoribonuclease. Exoribonuclease 2 (XRN2) is the human homologue of the Saccharomyces cerevisiae RAT1, which functions as a nuclear 5' to 3' exoribonuclease and is essential for mRNA turnover and cell viability. XRN2 also processes rRNAs and small nucleolar RNAs (snoRNAs) in the nucleus. XRN2 moves along with RNA polymerase II and gains access to the nascent RNA transcript after the endonucleolytic cleavage at the poly(A) site or at a second cotranscriptional cleavage site (CoTC). CoTC is an autocatalytic RNA structure that undergoes rapid self-cleavage and acts as a precursor to termination by presenting a free RNA 5' end to be recognized by XRN2. XRN2 then travels in a 5'-3' direction like a guided torpedo and facilitates the dissociation of the RNA polymerase elongation complex.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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