Product Detail
Product NameRND3 Antibody
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC, IHC, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesARHE antibody
memB antibody
Protein MemB antibody
Ras homolog gene family member E antibody
Rho family GTPase 3 antibody
Rho-related GTP-binding protein Rho8 antibody
Rho-related GTP-binding protein RhoE antibody
Rho8 antibody
RhoE antibody
Rnd3 antibody
RND3_HUMAN antibody
Small GTP binding protein Rho8 antibody
Accession NoSwiss-Prot#:P61587
Uniprot
P61587
Gene ID
390;
Calculated MW27 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC: 1:100-1:200
Western blot analysis of RND3 on human RND3 recombinant protein using anti-RND3 antibody at 1/1,000 dilution.
Western blot analysis of RND3 on HEK293 (1) and RND3-hIgGFc transfected HEK293 (2) cell lysate using anti-RND3 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RND3 antibody. Counter stained with hematoxylin.
ICC staining RND3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
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