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Location： Home > Mouse Monoclonal Antibodies > AOF1 Antibody

AOF1 Antibody#48421

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Product Detail

Product NameAOF1 Antibody

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Namesamine oxidase (flavin containing) domain 1 antibody
amine oxidase, flavin containing 1 antibody
AOF1 antibody
bA204B7.3 antibody
C6orf193 antibody
dJ298J15.2 antibody
Flavin-containing amine oxidase domain-containing protein 1 antibody
FLJ33898 antibody
FLJ34109 antibody
FLJ43328 antibody
KDM1B antibody
KDM1B_HUMAN antibody
LSD2 antibody
lysine (K)-specific demethylase 1B antibody
Lysine-specific demethylase 2 antibody
Lysine-specific histone demethylase 1B antibody
Lysine-specific histone demethylase 2 antibody

Accession NoSwiss-Prot#:Q8NB78

Uniprot Q8NB78

Gene ID 221656;

Calculated MW92 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:100-1:200
Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1,000 dilution.
Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.
ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
AOF1 (amine-oxidase flavin-containing domain 1), whose alternative names include KDM1B (lysine (K)-specific demethylase 1B) or LSD2 (lysine-specific histone demethylase 2), is an 823 amino acid nuclear protein belonging to the flavin monoamine oxidase family. As a histone demethylase, AOF1 specifically demethylates Lys4 of histone H3, a marker for epigenetic transcriptional activation, by interacting with a long stretch of the H3 N-terminal tail of mono- and dimethylated Lys4. Existing as three alternatively spliced isoforms, AOF1 is required for de novo DNA methylation of some imprinted genes in oocytes and contains a single SWIRM domain that is implicated in chromatin regulation. AOF1 contains one CW-type zinc finger and is encoded by a gene located on human chromosome 6p22.3.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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