Product Detail
Product NameRF1 Antibody
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesCl1 protein antibody
D5S1995 antibody
ERF antibody
eRF1 antibody
ERF1_HUMAN antibody
ETF1 antibody
Eukaryotic peptide chain release factor subunit 1 antibody
Eukaryotic release factor 1 antibody
Eukaryotic translation termination factor 1 antibody
MGC111066 antibody
Polypeptide chain release factor 1 antibody
Protein Cl1 antibody
RF1 antibody
Sup45 (yeast omnipotent suppressor 45) homolog like 1 antibody
SUP45L1 antibody
TB3 1 antibody
TB3-1 antibody
Accession NoSwiss-Prot#:P62495
Uniprot
P62495
Gene ID
2107;
Calculated MW49 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2,000
FC: 1:100-1:200
Western blot analysis of eRF1 on human eRF1 recombinant protein using anti-eRF1 antibody at 1/1,000 dilution.
Western blot analysis of eRF1 on HEK293 (1) and eRF1-hIgGFc transfected HEK293 (2) cell lysate using anti-eRF1 antibody at 1/1,000 dilution.
Western blot analysis of eRF1 on different cell lysate using anti-eRF1 antibody at 1/1,000 dilution. Positive control�� Lane1: MCF-7 Lane2: T47D Lane3: MOLT4 Lane4: Raji
Flow cytometric analysis of Hela cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Flow cytometric analysis of HepG2 cells with eRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Translation is carried out by the ribosome and several associated protein factors through three consecutive steps: initiation, elongation and termination. Termination of protein synthesis takes place when the ribosomal A site is occupied simultaneously by one of three stop codons and by a class 1 translation termination factor. In eukaryotes, this termination factor is the eukaryotic release factor 1 (eRF1), a protein that promotes hydrolysis of the last peptidyl-tRNA on the ribosome. eRF1 activity is stimulated by the association with the GTP-binding protein eRF3. eRF1 forms a quaternary complex with eRF3, GTP and the ribosome. This complex performs a dual role, where, in the "GTP state," it controls the positioning of eRF1 toward the stop codon and peptidyl-tRNA, and, in the "GDP state," it promotes the release of the eRFs from the ribosome. eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is essential for the interaction of eRF1 with the ribosome. The gene encoding human eRF1 maps to chromosome 5q31.2.
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