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PCDH9 Antibody#48425

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Product Detail

Product NamePCDH9 Antibody

Clone No.A2-G3

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesPCDH9 antibody
PCDH9_HUMAN antibody
Protocadherin-9 antibody

Accession NoSwiss-Prot#:Q9HC56

Uniprot Q9HC56

Gene ID 5101;

Calculated MW136 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000

ICC: 1:50-1:200

FC: 1:100-1:200
Western blot analysis of PCDH9 on human PCDH9 recombinant protein using anti-PCDH9 antibody at 1/1,000 dilution.
Western blot analysis of PCDH9 on HEK293 (1) and PCDH9-hIgGFc transfected HEK293 (2) cell lysate using anti-PCDH9 antibody at 1/1,000 dilution.
Western blot analysis of PCDH9 on C6 cell lysate using anti-PCDH9 antibody at 1/1,000 dilution.
ICC staining PCDH9 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PCDH9 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Potential calcium-dependent cell-adhesion protein.This gene encodes a member of the protocadherin family, and cadherin superfamily, of transmembrane proteins containing cadherin domains. These proteins mediate cell adhesion in neural tissues in the presence of calcium. The encoded protein may be involved in signaling at neuronal synaptic junctions. Sharing a characteristic with other protocadherin genes, this gene has a notably large exon that encodes multiple cadherin domains and a transmembrane region. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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