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EIF2A Antibody#48432

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Product Detail

Product NameEIF2A Antibody

Clone No.A8-A7

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC, IHC, FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names65 kDa eukaryotic translation initiation factor 2A antibody
CDA 02 antibody
CDA02 antibody
EIF 2 antibody
EIF 2A antibody
eIF-2A antibody
EIF2 antibody
eif2a antibody
EIF2A_HUMAN antibody
Eukaryotic translation initiation factor 2A 65kDa antibody
Eukaryotic translation initiation factor 2A antibody
MST089 antibody
MSTP004 antibody
MSTP089 antibody

Accession NoSwiss-Prot#:Q9BY44

Uniprot Q9BY44

Gene ID 83939;

Calculated MW65 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:50-1:200

FC: 1:50-1:100
Western blot analysis of EIF2A on human EIF2A recombinant protein using anti-EIF2A antibody at 1/1,000 dilution.
Western blot analysis of EIF2A on HEK293 (1) and EIF2A-hIgGFc transfected HEK293 (2) cell lysate using anti-EIF2A antibody at 1/1,000 dilution.
Western blot analysis of EIF2A on different cell lysate using anti-EIF2A antibody at 1/1,000 dilution. Positive control�� Line1: MCF-7 Line2: PC-12 Line3: HepG2 Line4: Hela Line5: Cos7 Line6: K562 Line7: Jurkat Line8: A431 Line9:
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-EIF2A antibody. Counter stained with hematoxylin.
ICC staining EIF2A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of HepG2 cells with EIF2A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
eIF2A (eukaryotic translation initiation factor 2A), also known as CDA02, MSTP004 or MSTP089, is a 585 amino acid protein that contains three WD repeats. Expressed ubiquitously with highest expression in heart, brain, pancreas and placenta, eIF2A functions as a translation initiation factor that binds Met-tRNA and directs it to 40S ribosomal subunits. Present in the early steps of protein synthesis, eIF2A controls the binding of Met-tRNA to 40S ribosomal subunits in a codon-dependent manner, in contrast to the eIF2 complex which accomplishes the same task in a GTP-dependent manner. In addition to its role in transcription initiation, eIF2A may also act to negatively regulate the expression of specific proteins, suggesting a possible role as a transcriptional repressor. eIF2A exists as two isoforms due to alternative splicing events.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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