Product Detail
Product NameTNNI2 Antibody
Clone No.A8-F12
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, IHC, FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesAMCD 2B antibody AMCD2B antibody DA 2B antibody DA2B antibody fast skeletal muscle antibody Fast twitch skeletal muscle troponin I antibody fast-twitch isoform antibody FSSV antibody fsTnI antibody TNNI 2 antibody Tnni2 antibody TNNI2_HUMAN antibody tro antibody Troponin I antibody Troponin I fast skeletal muscle antibody Troponin I fast twitch 2 antibody Troponin I fast twitch isoform antibody Troponin I fast twitch skeletal muscle isoform antibody Troponin I skeletal fast antibody Troponin I type 2 (skeletal fast) antibody Troponin I type 2 antibody
Accession NoSwiss-Prot#:P48788
Uniprot
P48788
Gene ID
7136;
Calculated MW21 kDa
Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000 FC: 1:100-1:200
IHC: 1:100-1:200
FC: 1:100-1:200
Western blot analysis of TNNI2 on human TNNI2 recombinant protein using anti-TNNI2 antibody at 1/1,000 dilution.
Western blot analysis of TNNI2 on HEK293 (1) and TNNI2-hIgGFc transfected HEK293 (2) cell lysate using anti-TNNI2 antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue using anti-TNNI2 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of NIH/3T3 cells with TNNI2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between actin and myosin by binding to calcium. Troponin is made up of at least two subunits, which are skeletal muscle skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator), and Troponin T (the link to the the regulator), and Troponin T C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has 2 isoforms: fast and slow. Fast troponin C has two calci um binding sites while slow / cardiac troponin C has a single calcium binding site.
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