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C-CBL Antibody#48473

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Product Detail

Product NameC-CBL Antibody

Clone No.C1-B12

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IHC,FC

Species ReactivityHu, Ms, Rt

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Names4732447J05Rik antibody
C CBL antibody
Cas Br M (murine) ecotropic retroviral transforming sequence antibody
Casitas B lineage lymphoma proto oncogene antibody
Casitas B-lineage lymphoma proto-oncogene antibody
CBL 2 antibody
cbl antibody
CBL_HUMAN antibody
CBL2 antibody
E3 ubiquitin protein ligase CBL antibody
E3 ubiquitin-protein ligase CBL antibody
Oncogene CBL2 antibody
Proto oncogene c CBL antibody
Proto-oncogene c-CBL antibody
RGD1561386 antibody
RING finger protein 55 antibody
RNF55 antibody
Signal transduction protein CBL antibody

Accession NoSwiss-Prot#:P22681

Uniprot P22681

Gene ID 867;

Calculated MW120 kDa

Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:100-1:200
Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1,000 dilution.
Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1,000 dilution.
Positive control:
Lane 1: RAJI
Lane 2: RAW264.7
Lane 3: K562
Lane 4: SKBR-3
Lane 5: 3T3-L1
Lane 6: THP-1
Lane 7: PC-12
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.
ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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