Product Detail
Product NameHSF4 Antibody
Clone No.A11-E7
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB,FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesCataract, Marner antibody
CTM antibody
Heat shock factor protein 4 antibody
Heat shock transcription factor 4 antibody
hHSF4 antibody
HSF 4 antibody
HSF4 antibody
HSF4_HUMAN antibody
HSTF 4 antibody
Accession NoSwiss-Prot#:Q9ULV5
Uniprot
Q9ULV5
Gene ID
3299;
Calculated MW53 kDa
Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
FC: 1:100-1:200
Western blot analysis of HSF4 on human HSF4 recombinant protein using anti-HSF4 antibody at 1/1,000 dilution.
Flow cytometric analysis of HeLa cells with HSF4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen, at both the mRNA and protein level. HSF1 is normally found as a monomer, whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. HSF4 exists as two splice variants and is expressed in heart, brain and skeletal muscle as a homotrimer. HSF4a does not contain a DNA-binding domain and inhibits the formation of HSF1 nuclear bodies, thus repressing HSF1 mediated transcription. HSF4b does contain a DNA-binding domain and colocalizes with HSF1 nuclear bodies after heat shock.
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