Product Detail
Product NamePINCH Antibody
Clone No.B9-G7
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB,ICC,FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other Names2310016J22Rik antibody
4921524A02Rik antibody
AI507642 antibody
AU021743 antibody
AW551584 antibody
C430041B13Rik antibody
LIM and senescent cell antigen like domains 1 antibody
LIM and senescent cell antigen-like-containing domain protein 1 antibody
LIM zinc finger domain containing 1 antibody
LIM-type zinc finger domains 1 antibody
Lims1 antibody
LIMS1_HUMAN antibody
Lims1l antibody
Particularly interesting new Cys His protein 1 antibody
Particularly interesting new Cys His protein antibody
Particularly interesting new Cys-His protein 1 antibody
PINCH 1 antibody
PINCH antibody
PINCH-1 antibody
PINCH1 antibody
Renal carcinoma antigen NY REN 48 antibody
Renal carcinoma antigen NY-REN-48 antibody
senescent cell antigen antibody
wu:fc32c03 antibody
zgc:112533 antibody
Accession NoSwiss-Prot#:P48059
Uniprot
P48059
Gene ID
3987;
Calculated MW37 kDa
Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
ICC: 1:100-1:500
FC: 1:100-1:200
Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution.
Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Jurkat
Lane 3: Hela
ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK.
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