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FUK Antibody#48477

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Product Detail

Product NameFUK Antibody

Clone No.E2-C3

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,FC

Species ReactivityHu, Ms, Rt, Monkey

Immunogen DescRecombinant protein

ConjugateUnconjugated

Accession NoSwiss-Prot#:Q8N0W3

Uniprot Q8N0W3

Gene ID 197258;

Calculated MW117 kDa, additional band 42kDa

Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000



FC: 1:100-1:200
Western blot analysis of Fucokinase on different cell lysates using anti- Fucokinase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Jurkat
Lane 4: A431
Lane 5: HEK293
Lane 6: MCF-7
Lane 7: PC-12
Lane 8: Cos7
Lane 9:NIH/3T3
Flow cytometric analysis of Hela cells with Fucokinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).
The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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