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INCENP Antibody#48478

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Product Detail

Product NameINCENP Antibody

Clone No.G9-D2

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IHC,FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other Namesbinds and activates aurora B and C in vivo and in vitro antibody
Chromosomal passenger protein antibody
INCE_HUMAN antibody
INCENP antibody
Inner centromere protein antibody
Inner centromere protein antigens 135/155kDa antibody
Inner centromere protein antigens 135kD 155kD antibody
Inner centromere protein INCENP antibody

Accession NoSwiss-Prot#:Q9NQS7

Uniprot Q9NQS7

Gene ID 3619;

Calculated MW105 kDa

Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:50-1:200
ICC: 1:100-1:500

FC: 1:100-1:200
Western blot analysis of INCENP on human INCENP recombinant protein using anti-INCENP antibody at 1/1,000 dilution.
Western blot analysis of INCENP on HEK293 (1) and INCENP-hIgGFc transfected HEK293 (2) cell lysate using anti-INCENP antibody at 1/1,000 dilution.
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-INCENP antibody. Counter stained with hematoxylin.
ICC staining INCENP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of Jurkat cells with INCENP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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