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GCNF Antibody#48479

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Product Detail

Product NameGCNF Antibody

Clone No.A10-G8

Host SpeciesMouse

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,ICC,IHC,FC

Species ReactivityHu

Immunogen DescRecombinant protein

ConjugateUnconjugated

Other NamesGCNF 1 antibody
GCNF antibody
GCNF1 antibody
Germ cell nuclear factor antibody
hGCNF antibody
hRTR antibody
NR 61 antibody
NR61 antibody
nr6a1 antibody
NR6A1_HUMAN antibody
Nuclear receptor subfamily 6 group A member 1 antibody
Orphan nuclear receptor NR6A1 antibody
Retinoic acid receptor related testis associated receptor antibody
Retinoid receptor related testis specific receptor antibody
Retinoid receptor-related testis-specific receptor antibody
RTR antibody

Accession NoSwiss-Prot#:Q15406

Uniprot Q15406

Gene ID 2649;

Calculated MW54 kDa

Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:1,000
IHC: 1:100-1:500
ICC: 1:50-1:200

FC: 1:100-1:200
Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1,000 dilution.
Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1,000 dilution.
Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1,000 dilution.
Positive control��
Lane1: K562
Lane2: NTERA-2
Lane3: HEK293
Lane4: HUVE-12
Lane5: HeLa
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.
ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants.

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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