Product Detail
Product NameNKX2.2 Antibody
Clone No.H12-E4
Host SpeciesMouse
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB,FC
Species ReactivityHu
Immunogen DescRecombinant protein
ConjugateUnconjugated
Other NamesHomeobox protein NK 2 homolog B antibody
Homeobox protein NK-2 homolog B antibody
Homeobox protein Nkx 2.2 antibody
Homeobox protein Nkx-2.2 antibody
NK 2 homolog B antibody
NK2 homeobox 2 antibody
NK2 transcription factor like protein B antibody
NK2 transcription factor related locus 2 antibody
NKX2 2 antibody
NKX2-2 antibody
NKX22 antibody
NKX22_HUMAN antibody
Nkx2b antibody
tinman antibody
Accession NoSwiss-Prot#:
Calculated MW30 kDa
Formulation1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:1,000
FC: 1:100-1:200
Western blot analysis of NKX2.2 on human NKX2.2 recombinant protein using anti-NKX2.2 antibody at 1/1,000 dilution.
Flow cytometric analysis of MCF-7 cells with NKX2.2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Members of the NK-2 family of homeodomain proteins are key regulators of growth and development in several tissues, including brain, heart and pancreas. During neural development, sonic hedgehog (Shh) is known to control cell fate and mitogenesis, which is correlated with Shh dose-dependent expression of several genes, including Nkx-2.1, Nkx-2.2 and Nkx-2.9. Specifically, the Nkx-2.2 protein is responsible for directing ventral neuronal patterning in response to graded Shh signaling. In the pancreas, Nkx-2.2 is expressed in a, b and pancreatic polypeptide (PP) cells, but not in d cells. Nkx-2.2 expression is required for differentiation of pancreatic b cells, which produce insulin. Homozygous null mutations of the Nkx-2.2 gene in mice lead to severe hyperglycemia and death shortly after birth, which suggests that Nkx-2.2 may be an important therapeutic target for pancreatic diseases, including diabetes and cancer.
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