Product Detail
Product NameNF-κB p65 Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationPeptide affinity purified
ApplicationsWB, IHC, FC
Species ReactivityHu, Ms, Rt
Immunogen DescThis antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal NF-κB p65.
ConjugateUnconjugated
Other NamesAvian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
MGC131774 antibody
NF kappa B p65delta3 antibody
NFKB3 antibody
Nuclear Factor NF Kappa B p65 Subunit antibody
Nuclear factor NF-kappa-B p65 subunit antibody
Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
OTTHUMP00000233473 antibody
OTTHUMP00000233474 antibody
OTTHUMP00000233475 antibody
OTTHUMP00000233476 antibody
OTTHUMP00000233900 antibody
p65 antibody
p65 NF kappaB antibody
p65 NFkB antibody
relA antibody
TF65_HUMAN antibody
Transcription factor p65 antibody
v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
V rel avian reticuloendotheliosis viral oncogene homolog A antibody
v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
Accession NoSwiss-Prot#:Q04206
Uniprot
Q04206
Gene ID
5970;
Calculated MW65 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:1,000-1:2,000
IHC: 1:100-1:200
FC: 1:50-1:100
Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1000 dilution. Positive control: Lane1: Hela Lane2:A549 Lane3: PC12 Lane 4: Mouse embryonic stem cell Lane5: NIH/3T3
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NF-��B p65 antibody. Counter stained with hematoxylin.
Flow cytometric analysis of Hela cells with NF-κB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.
If you have published an article using product 48498, please notify us so that we can cite your literature.
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et al,A multi-omics approach based on 1H-NMR metabonomics combined with target protein analysis to reveal the mechanism of RIAISs on cervical carcinomaIn Aging (Albany NY)On2022 Sep 27byChai Yanlan , Aibibai Jielili et al..PMID:36170024
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et al,Aurantio-obtusin Alleviates Dry Eye Disease by Targeting NF-κB/NLRP3 Signaling in Rodent Models
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et al,A multi-omics approach based on ¹H-NMR metabonomics combined with target protein analysis to reveal the mechanism of RIAISs on cervical carcinoma patients
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PMID: 36170024
et al,Identification of Cytochrome P450 2E1 as a Novel Target in Neuroinflammation and Development of Its Inhibitor Q11 as a Treatment Strategy.
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et al,Phellodendrine Exerts Protective Effects on Intra-abdominal Sepsis by Inactivating AKT/NF-kB Signaling.
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PMID: 39953352
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